The anti-inflammatory effects of Zingiber officinale ethanol extract on albumininduced inflammation in male Wistar albino rats were examined in this study. Rats were given low (100 mg/kg) and high (200 mg/kg) doses of Zingiber officinale extract after bovine serum albumin was used to promote inflammation. Serum levels of important pro-inflammatory cytokines, such as interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and interleukin-8 (IL-8), were used to measure the effects. Thirty rats were split up into five groups: two groups treated with Zingiber officinale, a blank control, a negative control, and a conventional control (treated with ibuprofen). When compared to the blank control (35.8 ± 0.024 pg/mL), high-dose Zingiber officinale extract dramatically decreased IL-1β levels (37.8 ± 0.000 pg/mL), demonstrating strong anti-inflammatory efficacy. Both the low-dose (2.71 ± 0.051 pg/mL) and high-dose (4.11 ± 0.0216 pg/mL) groups had lower levels of IL-6 than the control group (6.37 ± 0.004 pg/mL). TNF-α levels were significantly lower in the low-dose group (33.90 ± 0.017 pg/mL) than in the blank control group (44.90 ± 0.003 pg/mL). Nevertheless, IL-8 levels rose to 7.83 ± 0.027 pg/mL in all treated groups. These findings imply that Zingiber officinale has significant anti-inflammatory potential by downregulating TNF-α, IL-1β, and IL-6, especially at higher doses. The results demonstrated Zingiber officinale's potential as an affordable substitute for synthetic anti-inflammatory drugs and supported its therapeutic application in the management of inflammation.