Field experiment was conducted during the 2023 rainy season at Teaching and Research Farm of Faculty of Agriculture, Federal University of Kashere (Latitude 90 54’ 46’’N, Longitude 110 0’ 27’’E at 431 m above the sea level) Akko Local Government Area, Gombe State and at the Teaching and Research Farm of the Federal College of Horticulture Dadinkowa, Yamaltu-Deba Local Government Area, Gombe State. The treatments consisted of a factorial combination of three varieties of soybean (TGX-20D, TGX-1951 and TGX1448-2E) and three plant spacing of (20, 30 and 40 cm). These were laid out in a Randomized Complete Block Design (RCBD) with three replications. The results of the study showed that soybean variety TGX1448-2E significantly produced higher of (9.35 t/ha) yield than TGX-20D (9.04 t/ha) and TGX-1951 (9.03 t/ha). The 40 cm plant spacing, significantly enhanced yield of (9,868.332 kg) than 20 (8,415.833kg) and 30 cm plant spacing (9,133.333 kg). Therefore, TGX1448-2E and 40 cm plant spacing should be used by farmers who grow soybean based from their superior performances by producing higher growth and yield of soybean.