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Artificial Digestion Surveillance for Trichinella in Slaughtered Pigs and Farm Management Practices: A One Health Investigation of Zoonotic Risk in Kumasi, Ghana


Sr No:
Page No: 22-27
Language: English
Authors: Richard Kusi Boateng*1, Kwame Antwi Darkwa1, Prince Oduro Kwarteng1, Bright Arthur2
Affiliation: 1*School of Veterinary Medicine, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, 2School of Medical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
Received: 2026-09-08
Accepted: 2026-10-13
Published Date: 2026-10-26
Abstract:
Background. Trichinella infection is a foodborne zoonosis acquired mainly through undercooked pork. Direct evidence of infective larvae in pigs is scarce in Ghana, where earlier work relied largely on serology. Methods. In a cross-sectional study (April–September 2025), muscle from the tongue, diaphragm and masseter of 100 pigs slaughtered at three facilities in the Kumasi metropolis was pooled (10 g per pig) and examined for larvae by pepsin–HCl artificial digestion followed by stereomicroscopy. Management practices on 25 farms in five source communities were assessed with a semi-structured questionnaire and a direct-observation checklist. Results. Trichinella larvae were not detected in any of the 100 pigs (0/100; exact 95% CI 0–3.6%). The pigs were 62 females and 38 males of five breeds. Of the 25 farms, 23 (92%) used intensive and two (8%) semi-intensive systems; none was free-range. Environmental hygiene was rated best or better on 15 farms, good on nine and poor on one, and 23 farms (92%) reported regular deworming. Conclusions. No larvae were detected in pigs from predominantly confined, peri-urban production in Kumasi. With 100 pigs, the study can exclude only a prevalence above about 3.6%, and individual pigs were not traced to farms, so the husbandry findings are descriptive and do not explain the negative result. Targeted, larger surveillance of free-range pigs, where serological exposure has been reported, is the priority for Ghana.
Keywords: Trichinella; trichinellosis; zoonosis; One Health; artificial digestion; pigs; slaughterhouse surveillance; Ghana.

Journal: IRASS Journal of Multidisciplinary Studies
ISSN(Online): 3049-0073
Publisher: IRASS Publisher
Frequency: Monthly
Language: English

Artificial Digestion Surveillance for Trichinella in Slaughtered Pigs and Farm Management Practices: A One Health Investigation of Zoonotic Risk in Kumasi, Ghana